EFFECT OF ALANINE AND THREONINE ON THE SYNTHESIS OF SERINE BY LEUCONOSTOC MESENTEROIDES

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O'Barr, Thomas P. (Agricultural Research Service, Washington, D.C.). Effect of alanine and threonine on the synthesis of serine by Leuconostoc mesenteroides. J. Bacteriol. 86:1321–1325. 1963.—Growth of Leuconostoc mesenteroides in the absence of serine was inhibited by l-alanine and l-threonine. Varying the glycine concentration when l-alanine was present suggested a direct relationship between the molar ratio of these two amino acids and the amount of growth achieved by the test organism. Supplements of leucovorin did not reverse the effect of l-alanine upon growth. In experiments with washed cells of L. mesenteroides, both l-alanine and l-threonine reduced the amount of C14-labeled formate fixed into serine and the amount of glycine-1-C14 found in boiling-water extracts of the cells. Neither l-alanine nor l-threonine had any inhibitory effect upon the glycine-dependent disappearance of formaldehyde by acetone powders prepared from L. mesenteroides.

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