Effect of alkaloid arborinine and flavonoid rutin obtained of native plants from Bahia-Brazil on the viability and function of murine spleen and thymus cells in vitro. / Efeitos do alcalóide arborinina e do flavonóide rutina, extraídos de plantas nativas da Bahia, sobre funcionalidade e viabilidade de células de baço e timo murino in vitro.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Arborinine is an acridone alkaloid obtained from Erthela bahiensis, plant popularly used as diuretic, antidiabetic, antithermic and expectorant. Rutin is a citroflavonol obtained from Dimorphandra mollis a native medicinal plant from cerrado region in Bahia Brazil and has been described as anti-oxidative, anti-hemorrhagic and as a blood vessel protector. The present study has examined their effects on the viability and function in vitro of immune system cells in a murine model. Rat spleen and thymus cells were incubated with 10nM, 1M e 10M of each drug in the presence or absence of PWM, LPS or ConA mitogens. Cellular proliferation was analyzed by H3-thymidin uptake after 48 and 72 hours and viability was measured by flow cytometry using Anexin V and propidium iodide after 24 and 48 hours. IFN-γ and IL-10 were measured by ELISA in the supernatant culture after 48 and 72h. Our resuts with cells treated with arborinine and stimulated by ConA showed an inhibition of splenocytes proliferation in 48h (21%) and 72h (28-32%) and an increase on thymocytes proliferation (49%) in 48h; a decrease on IL-10 production by splenocytes (27%) in 48h and by thymocytes in 48h (20-38%) and 72h(25%); a decrease on IFN-γ production by splenocytes (2%) in 48h and 72h and by thymocytes (10-20%) in 72h. The resuts with cells treated with arborinine and stimulated by PWM showed inhibitory effect under splenocytes proliferation in 48h (9%) and enhancement of thymocytes proliferation (14%) after 48 hours. Apoptosis increased on splenocytes (54%) and thymocytes (19%) in 24h and decreased on thymocytes (20%) in 48h. It was also observed a decrease on IL-10 production by splenocytes (30%) after 48h and by thymocytes after 72h (23-54%), an increase on IFN-γ production by thymocytes in 48h (25%) and a decrease in 72h (27%). Data reffered to splenocytes treated with arborinine and stimulated with LPS demonstrated an apoptosis decrease(10%) and a proliferation enhancement (6%) in 48h and an IFN-γ decrease (25-30%) in 48 and 72h. The results of cells treated with rutin and stimulated with ConA showed an inhibition on the proliferation of splenocytes (11%) in 48h and of thymocytes (15%) in 72h and a decrease on IFN-γ production by spleenocytes and thymocytes in 48h. When cells were treated with rutin and stimulated with PWM, it was observed an increase on apoptosis of thymocytes in 24h (17%) and of spleenocytes in 48h (33%) It was also verified a small decrease on IFN-γ production by spleenocytes in 48h and by thymocytes in 72h. No changes occurred regarding the IL-10 levels of cells stimulated by these lectins. The results of spleenocytes treated with rutin and stimulated by LPS showed a decrease on apoptosis in 24h (20%) and an increase on IL-10 levels in 48h (11%). It was not found any statistically significant variation on necrosis rate of cells treated with arborinine or rutin neither changes on viability and function values in the absence of mitogenic stimulus. Our data suggests that as arborinine alkaloid as rutin flavonoid exerts diverse effects in some immunological parameters of spleen and thymus cells stimulated by mitogens without inducing necrosis.

ASSUNTO(S)

ifn-γ alkaloids arborinina arborinine flavonóides il-10 apoptosis rutina lymphoproliferation alcalóides ifn-γ imunologia linfoproliferação, apoptose rutin, mitogens mitógenos flavonoids il-10

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