Effect of Antimycin A on Photosynthesis of Intact Spinach Chloroplasts 1

AUTOR(ES)
RESUMO

Low concentrations (0.5-10 μm) of antimycin A were shown to increase the rate of CO2 fixation, O2 evolution and inorganic phosphate esterification in intact spinach (Spinacia oleracea) chloroplasts. The increase was highest when the light intensity was saturating. Stimulation was independent of the bicarbonate concentration and was accompanied by an enhancement in the synthesis of glycerate 3-phosphate with a decrease in dihydroxyacetone phosphate. The antibiotic decreased the Michaelis constant of the chloroplast but not of ribulose 1,5-diphosphate carboxylase for bicarbonate. It was suggested that antimycin A is affecting that portion (outer envelope) of the intact chloroplast which contains the enzyme mechanism for controlling the pace of CO2 fixation.

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