Effect of Ca2+ ions on plasmid transformation of Streptococcus lactis protoplasts.
AUTOR(ES)
von Wright, A
RESUMO
The effects of Mg2+ and Ca2+ ions on the efficiency of the plasmid transformation of lysozyme-treated Streptococcus lactis protoplasts were compared. A 33-megadalton plasmid, pLP712, coding for lactose fermentation and a 6.5-megadalton plasmid, pGB301, coding for erythromycin and chloramphenicol resistance were used as model plasmids, and S. lactis MG1614 was the recipient. Replacing Mg2+ with Ca2+ in the transformation buffer was found to increase transformant frequency more than 10-fold with both plasmids.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=291800Documentos Relacionados
- Transformation of Streptococcus lactis Protoplasts by Plasmid DNA †
- High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA.
- Transformation and fusion of Streptococcus faecalis protoplasts.
- Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts †
- Plasmid transformation of Streptococcus lactis protoplasts: optimization and use in molecular cloning.
