Effect of colony type and pH on surface charge and hydrophobicity of Neisseria gonorrhoeae.
AUTOR(ES)
Magnusson, K E
RESUMO
The effect of colonial variation and growth at pH 7.2 or pH 6.0 on the surface properties of Neisseria gonorrhoeae was assessed by the use of two-phase partitioning and hydrophobic interaction chromatography. Cells grown at pH 7.2 tended to be both hydrophobic and to possess a slight negative charge. Growth at pH 6.0 appeared to decrease hydrophobicity and to increase the negative surface charge. Possession of a series of outer membrane proteins, termed the colony opacity-associated proteins, did not appear to significantly affect charge or hydrophobicity. Piliated cells tended to have a higher negative charge than nonpiliated variants. They also tended to be less hydrophobic at pH 7.2, but became more hydrophobic at pH 6.0. The implications of these findings are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=414627Documentos Relacionados
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