Effect of Dibutyryl Cyclic AMP on the Induction of Epstein-Barr Virus in Hybrid Cells
AUTOR(ES)
Zimmerman, J. E.
RESUMO
Treatment of Epstein-Barr virus (EBV) negative somatic cell hybrids with 5′-iododeoxyuridine (IUdR) induced synthesis of EBV antigens and virus particles. When dibutyryl cAMP (Bt2-cAMP) was present in medium after exposure of cultures to IUdR, the incidence of cells synthesizing EBV early and virus capsid antigens was increased. The time necessary for appearance of EBV particles after induction by IUdR was significantly reduced in the presence of Bt2-cAMP. This enhancement was evident to a lesser degree with 3′:5′ cAMP than with Bt2-cAMP and did not occur with any other of the related compounds tested. The response observed was dose dependent. Untreated (no IUdR) EBV negative hybrid cells exposed to Bt2-cAMP also synthesized EBV antigens. The concentration of intracellular cAMP may act as one of the control mechanisms selecting for gene expression in this system.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356786Documentos Relacionados
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