Effect of Isotopic Label on Buoyant Density Determinations of Viral DNA in the Preparative Ultracentrifuge

AUTOR(ES)

Cassai, Enzo

RESUMO

The buoyant density of herpes simplex virus DNA may be significantly increased by isotopic labeling, particularly with 14C. The apparent increase in buoyant density may be readily calculated from the specific activity of the DNA and is easily demonstrated by isopycnic centrifugation in both the preparative and analytical centrifuge.

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