Effect of Leaf Extracts of Aloe arborescens Mill subsp. natalensis Berger on Growth of Trichophyton mentagrophytes
AUTOR(ES)
Fujita, Keisuke
RESUMO
The antifungal activity of a lyophilized powder containing aloe leaf homogenate (whole-leaf powder) against Trichophyton mentagrophytes was investigated. The minimal inhibitory concentration was 25 mg/ml by the agar dilution method, using Sabouraud glucose agar medium. At subinhibitory concentrations, the powder exerted its main effect on colony growth by prolongation of the lag phase and inhibition of growth rate. Homogenates of fresh whole leaf were filtered through Whatman GF/A paper, and the filtrate was dialyzed and concentrated by molecular filtration using an Amicon hollow-fiber dialyzer concentrator DC-2, and a powder containing components with molecular weights higher than 10,000 (high-molecular-weight component powder) was prepared by lyophilization. The minimal inhibitory concentrations against three strains of T. mentagrophytes were all 10 mg/ml. The inhibitory activity was fungicidal and was lost by heating at 100°C for 30 min. Both the whole-leaf powder and the high-molecular-weight component powder induced various morphological abnormalities in spores and hyphae by the inhibition of spore germination and development of hyphae.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=352416Documentos Relacionados
- The Relation between Photosynthesis, Respiration, and Crassulacean Acid Metabolism in Leaf Slices of Aloe arborescens Mill 1
- Progesterone Binding and Inhibition of Growth in Trichophyton mentagrophytes
- Progesterone binding and inhibition of growth in Trichophyton mentagrophytes.
- Effect of storage temperature on viability of Trichophyton mentagrophytes in infected guinea pig skin scales.
- TOTAL LIPID OF TRICHOPHYTON MENTAGROPHYTES
