Effect of ligand binding on the buoyant density of DNA in Nycodenz gradients.
AUTOR(ES)
Ford, T C
RESUMO
The effect of ligand binding upon the buoyant density of DNA in Nycodenz gradients has been studied using DNAs of differing base compositions. The effect of both intercalating ligands (ethidium bromide and proflavin) and non-intercalating ligands (distamycin A, DAPI and netropsin) has been studied. The binding of intercalating ligands to DNA has essentially no effect on the buoyant density of DNA in Nycodenz gradients. The non-intercalating ligands were found to increase the buoyant density of DNA in a base specific manner. The increase in buoyant density can be interpreted in terms of disruption of the hydration shell of the DNA molecule caused by the binding of the ligand along the minor groove of the DNA helix.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=318568Documentos Relacionados
- Variation in Escherichia coli buoyant density measured in Percoll gradients.
- Freeze-cutting of density gradients.
- Simple device for the fractionation of density gradients.
- Effect of Isotopic Label on Buoyant Density Determinations of Viral DNA in the Preparative Ultracentrifuge
- Isopycnic sedimentation of DNA in metrizamide: the effect of low concentrations of ions on buoyant density and hydration