Effect of low level laser therapy (LILT) on the protein secretion by endodontic sealers stimulated macrophages / "Efeito da terapia com laser em baixa intensidade (LILT) na produção de proteínas por macrófagos estimulados por cimentos endodônticos"
AUTOR(ES)
Lorena Ribeiro de Sousa
DATA DE PUBLICAÇÃO
2006
RESUMO
The endodontic therapy seeks the dental root canal biological sealing, depending on substances used in this process and patients defense immune factors. LILT has shown an anti-inflammatory activity, improving the periapical repair process. This in vitro study aimed to analyze the effect of LILT at the secretory activity of macrophages previously activated by interferon-gamma and lypopolisaccharide from E.coli, and stimulated by substances leached from three endodontic sealers (zinc oxide-eugenol based, resinous and calcium hydroxide-based). Cytotoxicity of these substances was assessed by the MTT test. Activated macrophages were stimulated by the substances or not (control) and then, irradiated or not (control) and the secretion of pro-inflammatory proteins (interleukin-1 b, tumor necrosis factor-a and matrix metalloproteinase-1) was analyzed by ELISA test. The LILT was performed using a GaAlAs laser (780 nm, 70 mW, focal spot of 4.0 mm2, 1.67 sec, 3 J/cm2). Two irradiations with 6 h-intervals were done. The data was compared by either ANOVA test or Friedmans test. The cell viabilities of controls and cells treated by the sealers were similar. Production of IL -1 b and TNF-a were observed. There was a high production of MMP-1. However, statistical differences were not observed amongst the groups. The irradiated groups presented results similar to those of non irradiated groups. Substances leached from the endodontic sealers are non cytotoxic at these experiments conditions . These substances, as well as the LILT, at the parameter used, were not able to change the secretion of MMP-1, IL-1 b e TNF-a by activated macrophages.
ASSUNTO(S)
macrophages endodontic sealers ifn-? lilt lps mmp-1 il-1 b tnf-a cimentos endodônticos lilt mmp-1 il-1 b tnf-a il-1 b macrófagos
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