Effect of Manganese and Zinc Ions on the Growth of Rhizobium

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Five strains, representing four species of Rhizobium, were grown in specially purified synthetic culture solutions with manganese or zinc ion concentrations ranging from 0 to 10−5m. Omission of Mn2+ restricted growth to 0.4 to 10% of that of controls, depending on the species. Growth was generally maximum with 10−6m Mn2+ and nearly so with 10−5m. Omission of Zn2+ restricted growth to 1 to 20% of that of controls. Growth was generally maximum with 10−7m Zn2+. The 10−5m level of Zn2+ was toxic to a variable degree to all species, suppressing the growth of one strain of R. meliloti to 49% of that of controls, and of R. phaseoli to only 0.4% of that of controls. A Mn2+ × Zn2+ interaction was also obtained as evidenced by a higher apparent Zn2+ requirement with 10−7m Mn2+ than with 10−6m Mn2+.

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