Effect of Naturally Occurring nif Reiterations on Symbiotic Effectiveness in Rhizobium phaseoli
AUTOR(ES)
Romero, David
RESUMO
Most naturally occurring strains of Rhizobium phaseoli possess reiteration of the nif genes. Three regions contain nitrogenase structural genes in strain CFN42. Two of these regions (a and b) have copies of nifH, nifD, and nifK, whereas the third region (c) contains only nifH. Strains containing mutations in either nif region a or nif region b had significantly diminished symbiotic effectiveness compared with the wild-type strain on the basis of nodule mass, total nitrogenase activity per plant, nitrogenase specific activity, total nitrogen in the shoot, and percentage of nitrogen. A strain containing mutations in both nif region a and nif region b was totally ineffective. These data indicate that both nif region a and nif region b are needed for full symbiotic effectiveness in R. phaseoli.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=202557Documentos Relacionados
- Amplification and deletion of a nod-nif region in the symbiotic plasmid of Rhizobium phaseoli.
- Effect of Sym Plasmid Curing on Symbiotic Effectiveness in Rhizobium fredii†
- Genetic rearrangements of a Rhizobium phaseoli symbiotic plasmid.
- Genetic Rearrangements of a Rhizobium phaseoli Symbiotic Plasmid
- Rhizobium phaseoli symbiotic mutants with transposon Tn5 insertions.