Effect of nitrogen limitation on cell growth, lipid accumulation and gene expression in Chlorella sorokiniana
AUTOR(ES)
Li, Ying Xia, Zhao, Feng Juan, Yu, Dong Dong
FONTE
Braz. arch. biol. technol.
DATA DE PUBLICAÇÃO
2015-06
RESUMO
The influence of nitrogen (N) limitation on the cell growth, chlorophyll content, intracellular lipid production and expression levels of two pathway genes in Chlorella sorokiniana was investigated in mixotrophic culture in this study. The maximum biomass concentration of C. sorokinianacultured in modified BG11 medium containing 3, 6, 9, 12 and 15 mM N were 1.60, 2.21, 2.74, 3.18 and 3.21 g/L of dry cell weight, respectively with maximum specific growth rate of 0.180, 0.198, 0.201, 0.203, 0.206 day−1 during culture with an initial N feed of 3, 6, 9, 12 and 15 mM in the first eight days, respectively. The maximum lipid content was 51 % with 3 mM N. However, the maximum lipid productivity of 0.00884 g L−1 day−1 was achieved with 6 mM N. Expression levels of accD (heteromeric acetyl-CoA carboxylase beta subunit) and accI (homomeric acetyl-CoA carboxylase) genes in C. sorokiniana were studied by real-time PCR. Increased expression levels of accD reflected the increased lipid content in stationary phase. In contrast, expression of the acc1 gene always remained low, showing that the gene might not be critical for lipid accumulation.
Documentos Relacionados
- Effect of iron limitation on growth, siderophore production, and expression of outer membrane proteins of Vibrio cholerae.
- Arabidopsis Seedling Growth, Storage Lipid Mobilization, and Photosynthetic Gene Expression Are Regulated by Carbon:Nitrogen Availability1
- Effects of Hypobaric and Hyperbaric Helium Atmospheres on the Growth of Chlorella sorokiniana
- Growth of Chlorella sorokiniana at Hyperbaric Oxygen Pressures
- Regulation of Glutamine Synthetase by Light and during Nitrogen Deficiency in Synchronous Chlorella sorokiniana1