Effect of Osmotic Stabilizers on 14CO2 Production by Bacteria and Blood
AUTOR(ES)
Zwarun, A. A.
RESUMO
Evolution of 14CO2 by whole blood as well as by Diplococcus pneumoniae, Haemophilus sp., Pseudomonas aeruginosa, Pseudomonas diminuta, and Streptococcus pyogenes was examined by using the BACTEC system. The control medium was JLI no. 6A culture vial containing 30 ml of enriched tryptic soy broth and 1.5 μCi of 14C-substrate. Hypertonic media consisted of control medium with either 1 or 3% NaCl, 10% sucrose, and 5%, 10%, or 15% dextran. The most deleterious treatment to bacteria was 3% NaCl since it not only retarded 14CO2 production, but also prevented growth of D. pneumoniae, Haemophilus, and P. diminuta. The 10% sucrose treatment diminished 14CO2 output, although it did not retard growth of test organisms. This effect was probably due to 14C-substrate dilution rather than to osmotic effects. Dextran had slight effect on 14CO2 production and slightly acidified the medium. Of the treatments tested, only 10% sucrose reduced normal output of 14CO2 by whole blood. This also is probably due to 14C-substrate dilution. It appears that 10% sucrose is potentially the most useful osmotic agent for radiometric techniques since, although bacterial 14CO2 production is lowered, blood 14CO2 is lowered also.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=380867Documentos Relacionados
- Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of 14CO2
- Effect of osmotic stabilizers on radiometric detection of cell wall-damaged bacteria.
- Effects of O2 Tension and Temperature during Light Uptake of 14CO2 on the Dark Release of 14CO2 by Excised C3 and C4 Leaves 1
- Photosynthetic 14CO2 Fixation by Green Cymbidium (Orchidaceae) Flowers
- The Effect of pH on the Products of Photosynthesis in 14CO2 by Chloroplast Preparations from Acetabularia mediterranea1