Effect of Oxygen on Photosynthesis by Spinach Leaf Protoplasts 1
AUTOR(ES)
Nishimura, Mikio
RESUMO
The photosynthetic CO2 fixation by spinach leaf (Spinacia oleracea L. var. Kyoho) protoplasts was inhibited by substituting an atmosphere of N2 with one of either air (21% O2) or 100% O2. The inhibitory effect of 100% O2 was greater than that of air. The mode of inhibition by 100% O2 and air was competitive with respect to CO2; Ki(O2) value was 0.32 mM at pH 7 and 0.28 mM at pH 8.5 The labeling patterns of compounds in protoplasts exposed to 14CO2 in light after transferring them from N2 to O2 atmospheres were examined. There was no detectable 14CO2 incorporation into glycolate under anaerobic and O2 atmospheres; a more marked labeling of glycine occurred under an oxidative environment compared to that under the anaerobic condition, presumably because of a rapid transformation of glycolate to glycine in the protoplasts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541906Documentos Relacionados
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