Effect of Phosphoenolpyruvate and Oxaloacetate on Ca2+ Uptake by Isolated Mung Bean Mitochondria 1
AUTOR(ES)
Graesser, Robert J.
RESUMO
Phosphoenolpyruvate partially inhibits the accumulation of Ca2+ in isolated mung bean (Phaseolus aureus Roxb.) mitochondria. Succinate-supported Ca2+ uptake is twice as sensitive to phosphoenolpyruvate inhibition as is NADH- or malate/pyruvate-supported Ca2+ uptake. Pyruvate, atractylate, and ATP, but not ITP, reverse the phosphoenolpyruvate-induced inhibition. Oxaloacetic acid inhibits succinate-supported Ca2+ uptake completely while partially inhibiting NADH-supported Ca2+ uptake. The oxaloacetate inhibition of NADH-supported Ca2+ uptake is greater than that produced by phosphoenolpyruvate. It is suggested that inhibition of Ca2+ uptake is due to the conversion of phosphoenolpyruvate into oxaloacetate via phosphoenolpyruvate carboxykinase, with oxaloacetate responsible for the actual inhibition of Ca2+ uptake.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=542348Documentos Relacionados
- Regulation of Malate Oxidation in Isolated Mung Bean Mitochondria: I. Effects of Oxaloacetate, Pyruvate, and Thiamine Pyrophosphate 12
- Effects of Guanidine Inhibitors on Mung Bean Mitochondria 1
- Effects of 2-Butylmalonate, 2-Phenylsuccinate, Benzylmalonate, and p-Iodobenzylmalonate on the Oxidation of Substrates by Mung Bean Mitochondria 1
- Store-operated Ca2+ entry depends on mitochondrial Ca2+ uptake
- ATP stimulates Ca2+ uptake and increases the free Ca2+ concentration in isolated rat liver nuclei.