Effect of Temperature Oscillation on Insect Cell Growth and Baculovirus Replication
AUTOR(ES)
Shao-Hua, Chang
FONTE
American Society for Microbiology
RESUMO
Temperature oscillation can enhance cell viability of sf9 insect cells and baculovirus production of occlusion bodies (OB) and extracellular virus (ECV) compared with constant temperature in stationary culture and suspension culture. The optimal oscillation range was 24 to 28°C. At this temperature oscillation, the viability of uninfected and infected sf9 cells can be maintained much longer than at 28°C. Although the rate of virus infection was a little low at 24 to 28°C, the final cell infectivity was similar to that at a constant temperature of 28°C. The production of OB was increased from 13.4 to 17.4/cell in stationary culture and from 13.9/cell to 18.1/cell in suspension culture. The titer of ECV was increased from 87 to 114 PFU/cell in stationary culture and from 79 to 114 PFU/cell in suspension culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=106305Documentos Relacionados
- Effect of temperature on spore germination and vegetative cell growth of Clostridium botulinum.
- Effect of Decreasing Growth Temperature on Cell Yield of Escherichia coli
- Effect of interferon, elevated temperature, and cell type on replication of acute hemorrhagic conjunctivitis viruses.
- Promoter influence on baculovirus-mediated gene expression in permissive and nonpermissive insect cell lines.
- Identification of baculovirus gene that promotes Autographa californica nuclear polyhedrosis virus replication in a nonpermissive insect cell line.
