Effect of the lambda S gene product on properties of the Escherichia coli inner membrane.
AUTOR(ES)
Wilson, D B
RESUMO
The S gene of bacteriophage lambda is a late gene required for cell lysis, but unlike the other two lysis genes, R and Rz, it does not code for an endolysin. Earlier studies have shown that the S gene product inhibits respiration and macromolecular synthesis and makes the inner membrane permeable to sucrose. In this study, the effect of the S gene product on a number of Escherichia coli membrane functions (active transport, permeability, respiration, and transhydrogenase and ATPase activity) were measured, and a product of the lambda S gene was identified in the inner membrane fraction by two-dimensional polyacrylamide gel electrophoresis. The results of these experiments indicate that the lambda S product is present in the inner membrane, that it increased the permeability of the membrane for all of the small molecules that were tested, and that its action is reversible. The simplest explanation of these results is that the S gene product forms a hydrophilic pore through the inner membrane, allowing small molecules and lambda lysozyme to pass through.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=220421Documentos Relacionados
- Oligomerization of the bacteriophage lambda S protein in the inner membrane of Escherichia coli.
- Substrate binding site for nitrate reductase of Escherichia coli is on the inner aspect of the membrane.
- Effects of ethanol on the Escherichia coli plasma membrane.
- Binding of pea cytochrome f to the inner membrane of Escherichia coli requires the bacterial secA gene product.
- Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane.