Effect of Thyroid Status on Insulin Action in Rat Adipocytes and Skeletal Muscle
AUTOR(ES)
Czech, Michael P.
RESUMO
Isolated adipocytes and soleus muscles prepared from mature rats, rendered hypothyroid by a low iodine diet and propylthiouracil, markedly resisted the ability of insulin to increase glucose utilization. In adipocytes, the sum of basal d-(1-14C)-glucose conversion to CO2, glyceride-glycerol, and fatty acid was unaltered by hypothyroidism, although conversion to fatty acid was decreased. The response of each of these metabolic pathways to insulin at all concentrations tested was greatly diminished in hypothyroid rat adipocytes. 3-O-Methylglucose transport rates in the presence of insulin were not significantly different in adipocytes from hypothyroid as compared with euthyroid rats, although basal transport rates were significantly higher in the hypothyroid state. Lipolysis and cyclic AMP accumulation in adipocytes from hypothyroid rats in response to theophylline were markedly diminished compared with euthyroid controls, but insulin was about as effective in inhibiting lipolysis in these cells as in those derived from euthyroid animals. The binding of 125I-insulin to adipocytes at several hormone concentrations was also shown to be unaffected by hypothyroidism.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=371686Documentos Relacionados
- Inhibitory Effect of Epinephrine on Insulin-stimulated Glucose Uptake by Rat Skeletal Muscle
- Insulin action on heart and skeletal muscle glucose uptake in essential hypertension.
- Effect of alterations in the thyroid state on the intrinsic contractile properties of isolated rat skeletal muscle
- Effect of Insulin on Protein Synthesis in Skeletal Muscle of an Isolated Perfused Preparation of Rat Hemicorpus
- Effects of fasting on insulin binding, glucose transport, and glucose oxidation in isolated rat adipocytes: relationships between insulin receptors and insulin action.