Effects of 2-Deoxy-d-Glucose and 3-Deazauridine Individually and in Combination on the Replication of Japanese B Encephalitis Virus
AUTOR(ES)
Woodman, Daniel R.
RESUMO
We have tested the potencies of the competitors of glucose, 2-deoxy-d-glucose, and of uridine, 3-deazauridine, on the inhibition of Japanese B encephalitis virus multiplication in BHK-21 cell cultures. The relative effectiveness of the viral inhibitors were evaluated individually and in combination in relation to cytotoxicity as a measure of the selectivity of inhibition. When the drugs were administered individually, the antiviral activity was masked by the cytotoxic effect on the host. By combining the two drugs, it was possible to inhibit Japanese encephalitis virus production at noncytotoxic concentrations. The effects of 2-deoxy-d-glucose and 3-deazauridine on the growth inhibition of BHK-21 cells in cultures were only additive, while they were clearly synergistic on the inhibition of Japanese encephalitis virus production. Thus, it was possible to achieve an increased antiviral effect without a significant increase in cytotoxicity. Although the precise biochemical mechanism of the antiviral activity of these antimetabolites in combination is not known, our results indicate the potential value of this approach in viral chemotherapy.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=352010Documentos Relacionados
- Effects of D-glucose, 2-deoxy-D-glucose and D-xylose on renal function in the rat.
- Dissociation of the inhibitory effects of 2-deoxy-D-glucose on Vero cell growth and the replication of herpes simplex virus.
- THE EFFECT OF 2-DEOXY-D-GLUCOSE INFUSIONS ON LIPID AND CARBOHYDRATE METABOLISM IN MAN*
- Inhibition of the Multiplication of Vesicular Stomatitis and Newcastle Disease Virus by 2-Deoxy-d-Glucose
- Increased Uptake of Amino Acids and 2-Deoxy-D-Glucose by Virus-Transformed Cells in Culture