Effects of methotrexate on glycosaminoglycan production by scleroderma fibroblasts in culture.
AUTOR(ES)
van den Hoogen, F H
RESUMO
OBJECTIVE--To determine the effects of increasing concentrations of methotrexate on the proliferation and glycosaminoglycan (GAG) synthesis of cultured dermal fibroblasts from patients with scleroderma. METHODS--Cultured dermal fibroblasts from nine patients with scleroderma and nine normal volunteers were grown for 72 hours in media containing various concentrations of methotrexate. The GAG synthesis in each cell was measured after incubating the fibroblasts with [3H]glucosamine and [35S]sulphate. RESULTS--A negative correlation was found between the concentration of methotrexate and numbers of fibroblasts from patients with scleroderma and normal controls. A positive correlation was found between GAG synthesis in each cell, as measured by [3H]glucosamine and [35S]sulphate incorporation, and increasing methotrexate concentrations in fibroblasts from patients with scleroderma and normal controls. CONCLUSIONS--These data indicate increased GAG synthesis in scleroderma and normal fibroblasts with increasing concentrations of methotrexate. Therefore the reported beneficial effect of methotrexate on skin fibrosis in scleroderma is most probably not the result of direct inhibition of GAG synthesis by fibroblasts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1005174Documentos Relacionados
- Simultaneous synthesis of types I and III collagen by fibroblasts in culture.
- The structure of acid mucopolysaccharides produced by Hurler fibroblasts in tissue culture.
- Production of Procollagen by Human Fibroblasts in Culture
- Effects of zinc-desferrioxamine on Plasmodium falciparum in culture.
- Effects of methyl isocyanate on rat brain cells in culture.