Effects of pH on the stability of chromatin core particles.

AUTOR(ES)

Libertini, L J

RESUMO

Chromatin core particles near physiological ionic strength undergo a reversible transition induced by changes in pH near neutrality. While sedimentation studies indicate no significant effect on size or shape, changes in tyrosine fluorescence anisotropy and in circular dichroism suggest a somewhat looser structure at high pH. Further support of this suggestion is given by high salt dissociation experiments; at pH 8 core particles begin to show changes at lower salt concentration than at pH 6. The pH transition appears unaffected by the presence of Mg2+ but can be blocked by crosslinking of the histones. A possible relationship is suggested between this transition and increases in intracellular pH which correlate with enhancement in several aspects of cellular activity including DNA replication.

Documentos Relacionados

• Reversibility of the low-salt transition of chromatin core particles.
• pH modification of the effects of detergents on the stability of enteric viruses.
• Effects of storage temperature and pH on the stability of eleven beta-lactam antibiotics in MIC trays.
• Effects of Environmental pH on the Internal pH of Chlorella pyrenoidosa, Scenedesmus quadricauda, and Euglena mutabilis1
• 1H NMR investigation of the conformational states of DNA in nucleosome core particles.