Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusion
AUTOR(ES)
Sousa, Renato Chavasco de, Moreira Neto, Antonio Augusto, Capelozzi, Vera Luíza, Ab'Saber, Alexandre Muxfeldt, Rodrigues, Olavo Ribeiro
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2015-05
RESUMO
PURPOSE: To investigate the effect of vardenafil in kidney of rats submitted to acute ischemia and reperfusion.METHODS:Twenty-eight rats were randomly distributed into two groups. Right nephrectomy was performed and the vardenafil group received vardenafil solution (at a concentration of 1 mg/ml in 10 mg/kg) while the control group received 0.9% saline solution (SS) one hour prior to the ligature of the left renal pedicle. After one hour of ischemia, animals were submitted to twenty-four hours of reperfusion, followed by left nephrectomy. The kidney's histological parameters evaluated on the study included vacuolar degeneration and tubular necrosis. Apoptosis was assessed by immunohistochemistry for cleaved caspase-3 using the point-counting and digital methods (Cytophotometry). Also, a biochemical analysis for creatinine was conducted.RESULTS: There were statistically significant differences between groups only with regards to the vacuolar degeneration parameter and to the cleaved caspase-3 digital method.CONCLUSION: Vardenafil showed a protective effect on the kidney of rats subjected to acute ischemia and reperfusion in this model
Documentos Relacionados
- Effects of immunosuppression with tacrolimus and mycophenolate mofetil on renal histology and function in single kidney rats submitted to ischemia and reperfusion
- Influence of chlorpromazine on renal histology of rats submitted to ischemia and reperfusion injury
- Cardioprotective effect of lipstatin derivative orlistat on normotensive rats submitted to cardiac ischemia and reperfusion
- Analysis of the effects of topical renal hypothermia on lung tissue after kidney ischemia and reperfusion in rats
- Effect of hyperbaric hepatic hyperoxia on the liver of rats submitted to intermittent ischemia/reperfusion injury