Efficient chromosomal transposition of a Tc1/mariner- like transposon Sleeping Beauty in mice
AUTOR(ES)
Horie, Kyoji
FONTE
The National Academy of Sciences
RESUMO
The presence of mouse embryonic stem (ES) cells makes the mouse a powerful model organism for reverse genetics, gene function study through mutagenesis of specific genes. In contrast, forward genetics, identification of mutated genes responsible for specific phenotypes, has an advantage to uncover novel pathways and unknown genes because no a priori assumptions are made about the mutated genes. However, it has been hampered in mice because of the lack of a system in which a large-scale mutagenesis and subsequent isolation of mutated genes can be performed efficiently. Here, we demonstrate the efficient chromosomal transposition of a Tc1/mariner-like transposon, Sleeping Beauty, in mice. This system allows germ-line mutagenesis in vivo and will facilitate certain aspects of phenotype-driven genetic screening in mice.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55396Documentos Relacionados
- Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells
- MTID: a database of Sleeping Beauty transposon insertions in mice
- Characterization of Sleeping Beauty Transposition and Its Application to Genetic Screening in Mice
- Enhancer trapping in zebrafish using the Sleeping Beauty transposon
- The DNA-bending protein HMGB1 is a cellular cofactor of Sleeping Beauty transposition
