Efficient Particle Production by Minimal Gag Constructs Which Retain the Carboxy-Terminal Domain of Human Immunodeficiency Virus Type 1 Capsid-p2 and a Late Assembly Domain
AUTOR(ES)
Accola, Molly A.
FONTE
American Society for Microbiology
RESUMO
The human immunodeficiency virus type 1 (HIV-1) Gag precursor Pr55gag by itself is capable of assembling into retrovirus-like particles (VLP). In the present study, we attempted to identify the minimal Gag sequences required for the formation of VLP. Our results show that about 80% of Pr55gag can be either deleted or replaced by heterologous sequences without significantly compromising VLP production. The smallest chimeric molecule still able to efficiently form VLP was only about 16 kDa. This minimal Gag construct contained the leucine zipper domain of the yeast transcription factor GCN4 to substitute for the assembly function of nucleocapsid (NC), followed by a P-P-P-P-Y motif to provide late budding (L) domain function, and retained only the myristylation signal and the C-terminal capsid-p2 domain of Pr55gag. We also show that the L domain function of HIV-1 p6gag is not dependent on the presence of an active viral protease and that the NC domain of Pr55gag is dispensable for the incorporation of Vpr into VLP.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=112023Documentos Relacionados
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