Eight Transfer RNAs Induced by Infection of Escherichia coli with Bacteriophage T4
AUTOR(ES)
McClain, William H.
RESUMO
Bacteriophage T4 induces the synthesis of eight transfer RNAs upon infection of E. coli. The tRNAs are easily detected and resolved into pure species by polyacrylamide gel electrophoresis of RNA labeled with 32P after T4 infection. Two-dimensional fingerprints of RNase T1 products derived from individual gel bands give patterns characteristic of single tRNAs. Furthermore, the T1 digest of each gel band has a single oligonucleotide that contains the minor nucleotides Tp and Ψp, a characteristic feature of all known tRNAs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389853Documentos Relacionados
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