Electric field-induced concentration gradients in planar supported bilayers.
AUTOR(ES)
Groves, J T
RESUMO
A simple method of generating electric field-induced concentration gradients in planar supported bilayers has been developed. Gradients of charged, fluorescently labeled probes were visualized by epifluorescence microscopy and could be observed at field strengths as low as 1 V/cm. Steady-state concentration gradients can be described by a simple competition between random diffusion and electric field-induced drift. A model based on this principle has been used to determine the diffusion coefficient of the fluorescent probes. This technique achieves a degree of electrical manipulation of supported bilayers that offers a variety of possibilities for the development of new molecular architectures and the study of biological membranes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1236430Documentos Relacionados
- Electric field-induced reorganization of two-component supported bilayer membranes
- Electric field-induced critical demixing in lipid bilayer membranes
- Electrotonic measurements by electric field-induced polarization in neurons: theory and experimental estimation.
- The effects of lyotropic anions on electric field-induced guidance of cultured frog nerves.
- Electrical manipulation of glycan-phosphatidyl inositol-tethered proteins in planar supported bilayers.