Electrochemical Gradients and K+ and Cl− Fluxes in Excised Corn Roots 12

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RESUMO

The compartmental analysis method was used to estimate the K+ and Cl− fluxes for cells of excised roots of Zea mays L. cv. Golden Bantam. When the measured fluxes are compared to those calculated with the Ussing-Teorell flux-ratio equation, an active inward transport of Cl− across the plasmalemma is indicated; the plasmalemma K+ fluxes are not far different from those predicted for passive diffusion, although an active inward transport cannot be precluded. Whether fluxes across the tonoplast are active or passive depends upon the vacuolar potential which is unknown. Assuming no electropotential gradient, the tracer flux ratios are fairly close to those predicted for passive movement. However, if the vacuole is positive by about 10 millivolts relative to the cytoplasm, the data suggest active inward transport for K+ and outward transport for Cl−.

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