Electrophoretic and Immunoelectrophoretic Studies of Sera from Normal, Tuberculous, and Noninfected Tuberculin-Sensitive Guinea Pigs1

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Dardas, T. J. (Michigan State University, East Lansing), and V. H. Mallmann. Electrophoretic and immunoelectrophoretic studies of sera from normal, tuberculous, and noninfected tuberculin-sensitive guinea pigs. J. Bacteriol. 92:76–81. 1966.—Normal guinea pig serum was separated into seven fractions by electrophoresis on cellulose acetate membranes. Thirty antigens were found by immunoelectrophoresis: albumin, 6 α1 globulins, 11 α2 globulins, 6 β1 globulins, 5 β2 globulins, and γ globulin. Hyper-α2-globulinemia was detected in sera from guinea pigs 14 days after inoculation with viable virulent Mycobacterium bovis. An additional α2 globulin, not demonstrable prior to infection, was detected concomitantly with the hyper-α2-globulinemia by immunoelectrophoresis. The additional α2 globulin was tentatively named α2-T. It persisted until the death of the guinea pigs. Neither hyper-α-globulinemia nor the α2-T antigen was detected by cellulose acetate electrophoresis and immunoelectrophoresis of sera from guinea pigs sensitized with heat-killed M. bovis. Both changes were due to the disease, not to delayed sensitivity alone.

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