Electrophoretic patterns of deadenylylated chorion and globin mRNAs.

AUTOR(ES)

Vournakis, J N

RESUMO

Treatment of silkmoth chorion mRNAs with calf thymus RNase H (EC 3.1.4.34; RNA-DNA-hybrid ribonucleotidohydrolase) in the presence of oligo(dT) specifically and effectively removes the 3'-terminal poly(A) sequences. Excision of non-poly(A) fragments cannot be detected. Under these conditions, RNase H leads to increased electrophoretic homogeneity of rabbit globin mRNA, presumably as a result of removal of poly(A) sequences that are inherently variable in length. Treatment with RNase H converts the three diffuse zones of messages for the several chorion proteins into multiple sharp bands.

Documentos Relacionados

• Patterns of expression of erythroblast non-globin mRNAs.
• Molecular evolution of human and rabbit beta-globin mRNAs.
• Structural and evolutionary analysis of the two chimpanzee alpha-globin mRNAs.
• The nucleotide sequences of the untranslated 5' regions of human alpha- and beta-globin mRNAs.
• Transcriptional analyses of interferon-inducible mRNAs.