Electroporation for the efficient transfection of mammalian cells with DNA.
AUTOR(ES)
Chu, G
RESUMO
A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=340526Documentos Relacionados
- Efficient DNA transfection of quiescent mammalian cells using poly-L-ornithine.
- Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.
- Electroporation-mediated transfection of Acholeplasma laidlawii with mycoplasma virus L1 and L3 DNA.
- Transfection of human lymphoblastoid cells with herpes simplex viral DNA.
- Efficient in situ electroporation of mammalian cells grown on microporous membranes.