Elimination of mRNA splicing by a point mutation outside the conserved GU at 5' splice sites.
AUTOR(ES)
Montell, C
RESUMO
Nearly all mRNA introns begin with the dinucleotide GU. Mutations in either of these virtually invariant bases have been found to inactivate the corresponding 5' splice site. Until now single base changes in neighboring bases have not been found to completely inactivate a 5' splice site. Here we show that a single A----U transversion in the third position of the adenovirus 2 E1A 13S mRNA intron does prevent RNA splicing at the corresponding 5' splice site.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=318792Documentos Relacionados
- The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5' splice sites.
- Nucleotide sequence at polyoma VP1 mRNA splice sites.
- A reappraisal of non-consensus mRNA splice sites.
- Neural network detects errors in the assignment of mRNA splice sites.
- The choice of alternative 5' splice sites in influenza virus M1 mRNA is regulated by the viral polymerase complex.