ELL2, a new member of an ELL family of RNA polymerase II elongation factors

AUTOR(ES)

Shilatifard, Ali

FONTE

The National Academy of Sciences of the USA

RESUMO

We recently isolated an RNA polymerase II elongation factor from rat liver nuclei and found it to be homologous to the product of the human ELL gene, a frequent target for translocations in acute myeloid leukemia. To further our understanding of the possible role(s) of ELL in transcriptional regulation and human disease, we initiated a search for ELL-related proteins. In this report we describe molecular cloning, expression, and characterization of human ELL2, a novel RNA polymerase II elongation factor 49% identical and 66% similar to ELL. Mechanistic studies indicate that ELL2 and ELL possess similar transcriptional activities. Structure–function studies localize the ELL2 elongation activation domain to an ELL2 N-terminal region that is highly homologous to ELL. Finally, Northern blot analysis reveals that the ELL2 and ELL genes are transcribed in many of the same tissues, but that the ratio of their transcripts exhibits tissue-to-tissue variation, raising the possibility that ELL2 and ELL may not perform completely general functions, but, instead, may perform gene- or tissue-specific functions.

Documentos Relacionados

• ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL
• dELL is an essential RNA polymerase II elongation factor with a general role in development
• RNA Polymerase II Elongation Factors of Saccharomyces cerevisiae: a Targeted Proteomics Approach
• Molecular cloning of an essential subunit of RNA polymerase II elongation factor SIII.
• RNA-directed DNA methylation requires an AGO4-interacting member of the SPT5 elongation factor family