Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer

AUTOR(ES)

Vázquez-Ibar, José Luis

FONTE

The National Academy of Sciences

RESUMO

Luminescence resonance energy transfer with a lanthanide like Tb3+ as donor is a useful technique for estimating intra- and intermolecular distances in macromolecules. However, the technique usually requires the use of a bulky chelator with a flexible linker attached to a Cys residue to bind Tb3+ and, for intramolecular studies, an acceptor fluorophor attached to another Cys residue in the same protein. Here, an engineered EF- hand motif is incorporated into the central cytoplasmic loop of the lactose permease of Escherichia coli generating a high-affinity site for Tb3+ (K\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{Tb}^{3+}}}\end{equation*}\end{document} ≈ 4.5 μM) or Gd3+ (K\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{Gd}^{3+}}}\end{equation*}\end{document} ≈ 2.3 μM). By exciting a Trp residue in the coordination sequence, Tb3+ bound to the EF-hand motif is sensitized specifically, and the efficiency of energy transfer to strategically placed Cys residues labeled with fluorophors is measured. In this study, we use the technique to measure distance from the EF-hand in the central cytoplasmic loop of lactose permease to positions 179 or 169 at the center or periplasmic end of helix VI, respectively. The average calculated distances of ≈23 Å (position 179) and ≈33 Å (position 169) observed with three different fluorophors as acceptors agree well with the geometry of a slightly tilted α-helix. The approach should be of general use for studying static and dynamic aspects of polytopic membrane protein structure and function.

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