Enhancer competition between H19 and Igf2 does not mediate their imprinting

AUTOR(ES)

Schmidt, Jennifer V.

FONTE

The National Academy of Sciences

RESUMO

The linked H19 and Igf2 genes on mouse distal chromosome 7 are subject to genomic imprinting. Competition between the promoters of the genes for transcription from shared enhancers has been proposed as an explanation for the coordinate expression and reciprocal imprinting of these two genes. To test this model, we have used Cre-loxP technology to generate in mice a conditional deletion of the H19 promoter and structural gene that leaves no transcription unit in the locus. Contrary to the prediction of enhancer competition we find that transcriptional activity from the H19 promoter is not required for the imprinted silencing of the Igf2 gene.

Documentos Relacionados

• Igf2 imprinting does not require its own DNA methylation or H19 RNA
• The Absence of Enhancer Competition between Igf2 and H19 following Transfer into Differentiated Cells
• Epigenetic changes encompassing the IGF2/H19 locus associated with relaxation of IGF2 imprinting and silencing of H19 in Wilms tumor.
• Deletion of the H19 differentially methylated domain results in loss of imprinted expression of H19 and Igf2
• DNA methylation modulates H19 and IGF2 expression in porcine female eye