Enterotoxin synthesis by nonsporulating cultures of Clostridium perfringens.
AUTOR(ES)
Goldner, S B
RESUMO
Chemostat-cultured Clostridium perfringens ATCC 3624 and NCTC 10240, and a nonsporulating mutant strain, 8-5, produced enterotoxin in the absence of sporulation when cultured in a chemically defined medium at a 0.084-h-1 dilution rate at 37 degrees C. The enterotoxin was detected by serological and biological assays. Examination of the chemostat cultures by electron microscopy did not reveal sporulation at any stage. The culture maintained enterotoxigenicity throughout cultivation in a continuous system. The enterotoxin was detected in batch cultures of each strain cultivated in fluid thioglycolate medium and a chemically defined medium. No heat-resistant or light-refractile spores were detected in batch cultures during the exponential growth.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=203547Documentos Relacionados
- Spore coat protein and enterotoxin synthesis in Clostridium perfringens.
- Enterotoxin formation by different toxigenic types of Clostridium perfringens.
- Measurement of biological activities of purified and crude enterotoxin of Clostridium perfringens.
- Metastatic endophthalmitis caused by Clostridium perfringens.
- Transformation of bile acids by Clostridium perfringens.