Enzymatic cleavage of a bacterial genome at a 10-base-pair recognition site.
AUTOR(ES)
Weil, M D
RESUMO
The circular genome of Staphylococcus aureus was cut into two fragments by a simple enzymatic method that cleaves a 10-base-pair site. The recognition sequence, A-T-C-G-mA decreases T-C-G-mA-T, was created by the combined use of the methylase M.Cla I (A-T-C-G-mA-T) and the restriction endonuclease Dpn I (G-mA decreases T-C). This technique is insensitive to CpG methylation and in human DNA is predicted to produce fragments that, on average, are greater than five million base pairs. The ability to create such long pieces of DNA should facilitate mapping of large, complex chromosomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=286401Documentos Relacionados
- Site-specific cleavage of DNA at 8- and 10-base-pair sequences.
- Enzymatic cleavage of a bacterial chromosome at a transposon-inserted rare site.
- Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence.
- An intronic 10-base-pair deletion in a class II A beta gene affects RNA processing.
- Purification of a yeast centromere-binding protein that is able to distinguish single base-pair mutations in its recognition site.