Enzymatic potential of heterotrophic bacteria from a neutral copper mine drainage
AUTOR(ES)
Costa, Bruna Zucoloto da, Rodrigues, Viviane Drumond, Oliveira, Valéria Maia de, Ottoboni, Laura Maria Mariscal, Marsaioli, Anita Jocelyne
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
2016-12
RESUMO
Abstract Copper mine drainages are restricted environments that have been overlooked as sources of new biocatalysts for bioremediation and organic syntheses. Therefore, this study aimed to determine the enzymatic activities (esterase, epoxide hydrolase and monooxygenase) of 56 heterotrophic bacteria isolated from a neutral copper mine drainage (Sossego Mine, Canaã dos Carajás, Brazil). Hydrolase and monooxygenase activities were detected in 75% and 20% of the evaluated bacteria, respectively. Bacterial strains with good oxidative performance were also evaluated for biotransformation of organic sulfides. Fourteen strains with good enzymatic activity were identified by 16S rRNA gene sequencing, revealing the presence of three genera: Bacillus, Pseudomonas and Stenotrophomonas. The bacterial strains B. megaterium (SO5-4 and SO6-2) and Pseudomonas sp. (SO5-9) efficiently oxidized three different organic sulfides to their corresponding sulfoxides. In conclusion, this study revealed that neutral copper mine drainages are a promising source of biocatalysts for ester hydrolysis and sulfide oxidation/bioremediation. Furthermore, this is a novel biotechnological overview of the heterotrophic bacteria from a copper mine drainage, and this report may support further microbiological monitoring of this type of mine environment.
Documentos Relacionados
- New medium for isolating iron-oxidizing and heterotrophic acidophilic bacteria from acid mine drainage.
- MICROORGANISMS IN ACID DRAINAGE FROM A COPPER MINE
- Acidophilic, Heterotrophic Bacteria of Acidic Mine Waters
- Characterization of the core microbiota of the drainage and surrounding soil of a Brazilian copper mine
- Hexavalent chromium reduction by aerobic heterotrophic bacteria indigenous to chromite mine overburden