Enzymatic switching for efficient and accurate translesion DNA replication

AUTOR(ES)

McCulloch, Scott D.

FONTE

Oxford University Press

RESUMO

When cyclobutane pyrimidine dimers stall DNA replication by DNA polymerase (Pol) δ or ε, a switch occurs to allow translesion synthesis by DNA polymerase η, followed by another switch that allows normal replication to resume. In the present study, we investigate these switches using Saccharomyces cerevisiae Pol δ, Pol ε and Pol η and a series of matched and mismatched primer templates that mimic each incorporation needed to completely bypass a cis–syn thymine–thymine (TT) dimer. We report a complementary pattern of substrate use indicating that enzymatic switching involving localized translesion synthesis by Pol η and mismatch excision and polymerization by a major replicative polymerase can account for the efficient and accurate dimer bypass known to suppress sunlight-induced mutagenesis and skin cancer.

Documentos Relacionados

• Mechanisms of accurate translesion synthesis by human DNA polymerase η
• Palm Mutants in DNA Polymerases α and η Alter DNA Replication Fidelity and Translesion Activity
• Efficient translesion replication in the absence of Escherichia coli Umu proteins and 3′–5′ exonuclease proofreading function
• Topoisomerase III is required for accurate DNA replication and chromosome segregation in Schizosaccharomyces pombe
• The β subunit sliding DNA clamp is responsible for unassisted mutagenic translesion replication by DNA polymerase III holoenzyme