Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples.
AUTOR(ES)
McIntosh, K
RESUMO
An enzyme-linked immunosorbent assay (ELISA) for respiratory syncytial virus antigens was applied to the rapid diagnosis of acute infections in children and was compared with viral culture and immunofluorescence tests. The ELISA test employed commercially available reagents and was run on a day-to-day basis as specimens were received in the laboratory. Sensitivity and specificity by ELISA were 82 and 95%, respectively, compared with culture. In the same specimens, the sensitivity and specificity by immunofluorescence were 86 and 96%, respectively. Nasopharyngeal aspirates were proven to be a better source of viral antigen than were nasopharyngeal swabs. ELISA-positive samples remained positive even when left unrefrigerated for a week or mailed to the laboratory in plastic containers. Respiratory syncytial virus ELISA, like culture, became negative as the disease progressed and showed no superiority over culture for diagnosis late in the illness.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=272355Documentos Relacionados
- Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: development and description.
- Enzyme-linked immunosorbent assay for measurement of serological response to respiratory syncytial virus infection.
- Production of antiserum to respiratory syncytial virus polypeptides: application in enzyme-linked immunosorbent assay.
- Simplified enzyme-linked immunosorbent assay for specific antibodies to respiratory syncytial virus.
- Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay.