Enzyme-linked immunosorbent assay for Salmonella typhimurium in food: feasibility of 1-day Salmonella detection.
AUTOR(ES)
Lee, H A
RESUMO
A microtitration plate, antibody-capture, enzyme-linked immunosorbent assay was developed for detection of Salmonella typhimurium. The assay utilizes a monoclonal detector antibody which shows no cross-reactions with non-Salmonella species and only a slight cross-reaction with one other Salmonella serotype. By using only one cultural stage (in a nonselective, chemically defined medium) prior to the enzyme-linked immunosorbent assay, low numbers of cells in food (10 cells 25 g-1) were detected in 19 h. Non-Salmonella competing organisms did not interfere with detection of S. typhimurium even when present in the ratio of 10(6):1 (non-Salmonella/Salmonella spp.). The assay shows the feasibility of rapid, 1-day testing for Salmonella spp. with antibody technology.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=184468Documentos Relacionados
- Further studies on the feasibility of one-day Salmonella detection by enzyme-linked immunosorbent assay.
- Competitive enzyme-linked immunosorbent assay for cholera-related enterotoxins in Salmonella typhimurium.
- Enzyme-linked immunosorbent assay for detection of Salmonella typhi protein antigen.
- Enzyme-linked immunosorbent assay spin amplification technique for herpes simplex virus antigen detection.
- Enzyme-linked immunosorbent assay for detection of Salmonella lipopolysaccharide in poultry specimens.
