Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli.
AUTOR(ES)
Sarkar, S
RESUMO
When a shuttle vector containing a tyrosine suppressor tRNA (supF) gene as a target for mutagenesis replicated in a monkey kidney cell line, the frequency of SupF+ mutations was 2.3 +/- 0.5 x 10(-3). When the host cells were treated with ethyl methanesulfonate 40 h before transfection, a 10-fold increase in SupF+ mutation frequency was observed. These results supported the hypothesis that a damage-inducible mutagenic pathway exists in mammalian cells and also demonstrated the utility of this shuttle vector for the study of mutagenesis in mammalian cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=369043Documentos Relacionados
- Inducible error-prone repair in Escherichia coli.
- Induction of error-prone repair as a consequence of DNA ligase deficiency in Escherichia coli.
- Multiple point mutations in a shuttle vector propagated in human cells: evidence for an error-prone DNA polymerase activity.
- One-step random mutagenesis by error-prone rolling circle amplification
- Mutagenesis of Neisseria gonorrhoeae: absence of error-prone repair.