Escherichia coli K-12 tolF mutants: alterations in protein composition of the outer membrane.
AUTOR(ES)
Chai, T J
RESUMO
Outer membrane materials prepared from three independently isolated spontaneous Escherichia coli tolF mutants contained no detectable protein Ia. The loss of this protein was nearly completely compensated for by an increase in other major outer membrane proteins, Ib and II. Thus, the major outer membrane proteins accounted for 40% of the total cell envelope protein in both tol+ and tolF strains. No changes were found in the levels of inner membrane proteins prepared from tolF strains when compared with similar preparations from the tol+ strain. Phage-resistant mutants were selected starting with a tolF strain by using either phage TuIb or phage PA2. These phage-resistant tolF strains contained neither protein Ia nor protein Ib. The mutation leading to the loss of protein Ib in these strains is independent of the tolF mutation and is located near malP on the E. coli genetic map.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=235281Documentos Relacionados
- New major outer membrane proteins found in an Escherichia coli tolF mutant resistant to bacteriophage TuIb.
- Two mutations which affect the barrier function of the Escherichia coli K-12 outer membrane.
- Assembly of TolC, a Structurally Unique and Multifunctional Outer Membrane Protein of Escherichia coli K-12
- Role of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12.
- Transcriptional regulation of Escherichia coli K-12 major outer membrane protein 1b.