Escherichia coli strains carrying the cloned cytochrome d terminal oxidase complex are sensitive to near-UV inactivation.
AUTOR(ES)
Sammartano, L J
RESUMO
To determine if membrane-bound cytochromes function as endogenous near-UV photosensitizers, strains containing the cloned cydA and cydB genes were tested for near-UV sensitivity. A strain containing both cloned genes overproduced cytochromes b558, b595, and d. Another strain containing only cloned cydB overproduced cytochrome b558. Both cytochrome-overproducing strains were hypersensitive to broad-spectrum near-UV inactivation. The presence of excess cytochromes did not affect sensitivity to far-UV radiation and provided protection against H2O2 inactivation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213941Documentos Relacionados
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