EspectrÃmetro de plÃsmons de superfÃcie no infravermelho para o desenvolvimento de biosensores

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

The development of sensors based on surface plasmon oscillations (SP) in metals is based on the high sensitivity of these oscillations to small fluctuations in the optical properties of the medium adjacent to the metallic surface. It is also possible through the characterization of these oscillations to determine optical properties and thicknesses of thin films deposited on the metal, as well as the topography of the metallic surface in submicrometer scale. Besides these applications, this technique has been used for the development of biological sensors capable to detect, quantify and monitor, in real time, the interaction between antigen and antibody molecules nearby a metallic surface. In this work, we first developed a pilot spectrometer in the visible region using a HeNe laser to observe and characterize the kinetics of biological reactions in aqueous media. Based on theoretical results published in the literature, that show a sensitivity increase in the surface plasmon resonance (SPR) by factor a of 10 through the use of light in the IR (λ ≈ 1 μm), we implemented an optical system at λ = 0,9751 μm for real-time monitoring of 25 reaction cells. Through this system it was possible to obtain the first measurements of SPR at λ=0,9751 μm ever reported in the literature and to establish the methodology of using this system for the development of high sensitivity biosensores

ASSUNTO(S)

biossensores engenharia eletrica plÃsmons de superfÃcie fotÃnica

ACESSO AO ARTIGO

Documentos Relacionados