Estimation of membrane potentials of individual lymphocytes by flow cytometry.
AUTOR(ES)
Shapiro, H M
RESUMO
The membrane potentials of individual cells can be estimated by flow cytometric quantitation of the cells' uptake of the fluorescent lipophilic cationic dye 3,3'-dihexyloxacarbocyanine iodide. Human lymphocytes separated from peripheral blood on Hypaque-Ficoll gradients are uniformly depolarized by gramicidin and hyperpolarized by valinomycin. Concanavalin A and phytohemagglutinin depolarize only a fraction of the lymphocytes. The flow cytometric technique allows precise detection of heterogeneous membrane potential responses to stimuli such as lectins; it could also provide a basis for sorting cells that respond differently to a given stimulus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411723Documentos Relacionados
- Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry.
- Rapid determination of antifungal activity by flow cytometry.
- Candida albicans serotype analysis by flow cytometry.
- Flow Cytometry. First Principles
- Detection and quantification of live, apoptotic, and necrotic human peripheral lymphocytes by single-laser flow cytometry.
