Estudo comparativo entre faseolamina comercial e farinha de feijÃo como perspectiva ao tratamento da obesidade e do diabetes mellitus tipo 2. / Comparative study between commercial phaseolamine and bean flour as a perspective to the treatment of obesity and type 2 diabetes mellitus.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Use of plants of medicinal purposes represents a wide field of research. New therapeutic proposals need studies to evaluate the appropriate use as to posology, indication, limitations and risks of utilization. The search for new phytotherapeutic ones as an alternative to the traditional medicinal treatment of obesity and of the type 2 diabetes mellitus has been growing. An example of that is bean (Phaseolus vulgaris L.), one of the most important components of the BrazilianÂs food diet and raw matter for the obtaining of the phytotherapeutic one phaseolamine (inhibitor of α-amylase), indicated for weight loss and to decrease glucemia for the decrease of the absorption of ingest starch. The objective in this work was to conduct a comparative chemical study between commercial phaseolamine and four bean cultivars and to evaluate the effects due to its use. In this way, the chemical constituents (centesimal composition and anti nutrients) and inhibition of digestive enzymes (α-amylase and trypsine) wee compared in the bean flours and in phaseolamine. No significant differences were found in the amounts of trypsine and α-amylase inhibitor and in the contents of protein, NFE, ether extract and in the investigated anti-nutrients (saponin and lectin) between bean flours and phaseolamine. These results furnish clues that there was no purification of α amylase inhibitor in the commercial sample. There was, nevertheless, a greater similarity between phaseolamine and white bean. Therefore, for the heat inactivation assays and biological assay, white bean was the chosen one. For verification of heat inactivation, the crude protein extracts obtained from extraction in water 1:10 were treated at 95ÂC for 20 minutes. The inhibition analysis after electrophoresis run showed that the trypsin inhibitor was thermally stable while the α-amylase inhibitor was inactive. So, the thermal treatment applied with the purpose of denaturing trypsin inhibitor and of preserving α-amylase inhibitor was no efficient. For evaluation of the biological effect, white bean extracts and phaseolamine were administered via oral (gavage) in mice for 24 days. The applied dose was the one indicated in the therapeutics (1g/70Kg/day) to each animal, a control group was given distilled water. No alterations were found in body weight, development, liver weight, small intestine and pancreas. The biochemical serum indices of AST, ALT and amylase also did not undergo alteration in relation to the control group. At the dose and administration time used, neither deleterious effects nor expected pharmacological activity were found.

ASSUNTO(S)

phaseolus vulgaris. 2. faseolamina. 3. antinutrientes. 4. diabetes 1.phaseolus vulgaris. 2. phaseolamine 3. antinutrients. 4. diabetes bioquimica

ACESSO AO ARTIGO

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