"Estudo da atividade biológica da macroprolactina humana em células Nb2 e em células Ba/F-03 transfectadas com o receptor de prolactina humano forma longa" / Human macroprolactin biological activity study in Nb2 cells and in Ba/F-03 cells expressing human long prolactin receptor

AUTOR(ES)

Andrea Glezer

DATA DE PUBLICAÇÃO

2006

RESUMO

Macroprolactinemia is a frequent finding in hyperprolactinemic individuals, usually without clinical impact. Data on biological activity of macroprolactin (bbPRL) is mostly based on a heterologous bioassay (Nb2 cell). Biological activity of bbPRL observed in vitro but not in vivo maybe due to its high molecular weight preventing its passage through capillary barrier. Alternatively, bbPRL bioactivity may differ depending on the PRL receptor species specificity. BbPRL from macroprolactinemic individuals and monomeric PRL (mPRL) from hyperprolactinemic patients without macroprolactinemia were tested in two bioassays: Nb2 and in Ba/F-LLP, which expresses human prolactin receptor. While both bioassays achieve similar results considering mPRL activity, our results indicate that bbPRL displays activity in a heterologous but not in a homologous bioassay, consistently with the apparent absence of bbPRL bioactivity in vivo

ASSUNTO(S)

receptores da prolactina receptors prolactin biological assay/methods protein isoforms prolactinoma prolactin prolactina bioensaios/métodos hyperprolactinemia isoformas de proteínas hiperprolactinemia prolactinoma

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