Estudo da expressão dos genes HOXB13 e HHEX em carcinomas epidermóides de boca através das técnicas de RT-PCR e Hibridização in situ. / HOXB13 and HHEX expression study in oral squamous cell carcinoma with the RT-PCR and in situ Hybridization techniques.

AUTOR(ES)

Claudia Cazal

DATA DE PUBLICAÇÃO

2004

RESUMO

The aim of this study was to verify the HOXB13 and HHEX genes expression in oral squamous cell carcinoma (OSCC) using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and in situ Hybridization techniques. Tumoral tissues and adjacent non-tumoral oral mucosa specimens were obtained from 30 patients with OSCC at the Head and Neck Surgery Service HC (FMUSP). The samples were cDNA extracted and submitted to the RT-PCR technique. The amplicons were visualized in electrophoresis on a 1% agarose gel with ethidium bromide. Genes expressions were correlated with UICC staging, TNM stage, tumor location, tumor thickness, adjacent tissues involvement, vascular and perineural invasion, and cellular differentiation. Finally, direct sequence analysis was performed on PCR products to confirm cDNA sequence. Riboprobes were confectioned for in situ hybridization analysis. RT-PCR results showed HOXB13 and HHEX transcripts in both tumoral and non-tumoral tissue samples; no statistical correlation was verified between HOXB13/HHEX expressions and tumoral or non-tumoral tissues; there was a positive correlation between HOXB13 tumoral expression and tumor thickness, neural invasion, and vascular invasion; there was a positive correlation between HHEX tumoral expression and adjacent tissue involvement, vascular invasion, and a inverse relationship with patients age; ISH technique exhibited a consistent and invariable pattern of expression for both genes on both tumoral and non-tumoral tissue samples. Present results points out to a correlation between HOXB13 and HHEX expressions, and some important prognostic indicators, and this may represent a valuable tool to understand the biological behavior of OSCC.

ASSUNTO(S)

carcinoma epidermóide in situ hybridization squamous cell carcinoma oral cancer câncer de boca hhex rt-pcr homeobox hoxb13 rt-pcr hhex hibridização in situ homeobox hoxb13

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