Estudo da proliferação e diferenciação de células-tronco hematopoéticas provenientes de sangue de cordão umbilical na presença e ausência de mitógenos. / Proliferation and differentiation study of hematopoetic stem cells from umbilical cord blood in the presence and absence of mitogens.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Hematopoietic stem cells (HSC) from umbilical cord blood (UCB) have a great potencial for hematopoietic reconstitution. Although these stem cells have been well characterized by their functional and fenotipics properties, self-renewal regulation and genes involved are still unknown. Analyses of cell growth, clonogenic assay and flow cytometry revealed the expansion and differentiation of HSC grown in medium with or without suplementation of the mitogens estradiol and LiCl. Expression of the subunit of telomerase increased in all treatments. As well as the expression of Nanog and Oct4, related to plutipotency and self-renewal. Nanog, Oct4, Sox2 and FoxD3 expression was also high in CD133 cells, in CD3 cells from peripherical blood and in clonogenic assay derived cells. Conclusion: medium without the suplementation is sufficient for expansion of HSC, keeping their characteristcs, realted to proliferation, self-renewal and pluripotency.

ASSUNTO(S)

cordão umbilical cell proliferation estradiol expressão gênica proliferação celular cord blood stem cells estradiol lithium chlorite gene expression células-tronco cloreto de lítio

ACESSO AO ARTIGO

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